Proteostasis Environment Shapes Higher-Order Epistasis Operating on Antibiotic Resistance.

ABSTRACT: Recent studies have affirmed that higher-order epistasis is ubiquitous and can have large effects on complex traits. Yet, we lack frameworks for understanding how epistatic interactions are influenced by central features of cell physiology. In this study, we assess how protein quality control machinery-a critical component of cell physiology-affects epistasis for different traits related to bacterial resistance to antibiotics. Specifically, we disentangle the interactions between different protein quality control genetic backgrounds and two sets of mutations: (i) SNPs associated with resistance to antibiotics in an essential bacterial enzyme (dihydrofolate reductase, or DHFR) and (ii) differing DHFR bacterial species-specific amino acid background sequences (Escherichia coli, Lis

SUBMITTER: Guerrero RF 

PROVIDER: S-EPMC6553834 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature