Potent neutralizing antibodies elicited by dengue vaccine in rhesus macaque target diverse epitopes.

ABSTRACT: There is still no safe and effective vaccine against dengue virus infection. Epidemics of dengue virus infection are increasingly a threat to human health around the world. Antibodies generated in response to dengue infection have been shown to impact disease development and effectiveness of dengue vaccine. In this study, we investigated monoclonal antibody responses to an experimental dengue vaccine in rhesus macaques. Variable regions of both heavy chain (VH) and light chain (VL) were cloned from single antibody-secreting B cells. A total of 780 monoclonal antibodies (mAbs) composed of paired VH and VL were characterized. Results show that the vaccination induces mAbs with diverse germline sequences and a wide range of binding affinities. Six potent neutralizing mAbs were identified amon

SUBMITTER: Li L 

PROVIDER: S-EPMC6553876 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature