Areca alkaloids measured from buccal cells using DART-MS serve as accurate biomarkers for areca nut chewing.

ABSTRACT:

Background

Areca nut (AN) chewing is carcinogenic and biomarkers reflecting it are urgently needed to determine the effectiveness of emergent cessation programs. Buccal cells (BCs) may serve as an ideal matrix to measure such biomarkers; however, their utility for this purpose is unknown. Direct analysis in real time-mass spectrometry (DART-MS) is a sensitive technique that analyzes materials in the open air and requires minimal/no sample preparation. We utilized DART-MS to analyze BCs to test the usefulness of this method in measuring areca alkaloids as biomarkers for AN chewing.

Methods

We applied DART-MS in positive-ion mode to quantitate over time human BCs: (a) exposed ex vivo to betel quid extracts (BQE) consisting of young AN, Piper betle L. leaf, slaked lime, and tob

SUBMITTER: Franke AA 

PROVIDER: S-EPMC6554057 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature