Exonuclease requirements for mammalian ribosomal RNA biogenesis and surveillance.

ABSTRACT: Ribosomal RNA (rRNA) biogenesis is a multistep process requiring several nuclear and cytoplasmic exonucleases. The exact processing steps for mammalian 5.8S rRNA remain obscure. Here, using loss-of-function approaches in mouse embryonic stem cells (mESCs) and deep sequencing of rRNA intermediates, we investigate the requirements of exonucleases known to be involved in 5.8S maturation at nucleotide resolution and explore the role of the Perlman syndrome-associated 3'-5' exonuclease Dis3l2 in rRNA processing. We uncover a novel cytoplasmic intermediate that we name '7S_B' rRNA that is generated through sequential processing by distinct exosome complexes. 7S_B rRNA can be oligoadenylated by an unknown enzyme and/or oligouridylated by TUT4/7 and subsequently processed by Di

SUBMITTER: Pirouz M 

PROVIDER: S-EPMC6554070 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature