Quantifying Protein Copy Number in Super Resolution Using an Imaging-Invariant Calibration.

ABSTRACT: The use of super-resolution microscopy in recent years has revealed that proteins often form small assemblies inside cells and are organized in nanoclusters. However, determining the copy number of proteins within these nanoclusters constitutes a major challenge because of unknown labeling stoichiometries and complex fluorophore photophysics. We previously developed a DNA-origami-based calibration approach to extract protein copy number from super-resolution images. However, the applicability of this approach is limited by the fact that the calibration is dependent on the specific labeling and imaging conditions used in each experiment. Hence, the calibration must be repeated for each experimental condition, which is a formidable task. Here, using cells stably expressing dynein intermediat

SUBMITTER: Cella Zanacchi F 

PROVIDER: S-EPMC6554488 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature