Redox properties and human serum albumin binding of nitro-oleic acid.

ABSTRACT: Nitro-fatty acids modulate inflammatory and metabolic stress responses, thus displaying potential as new drug candidates. Herein, we evaluate the redox behavior of nitro-oleic acid (NO₂-OA) and its ability to bind to the fatty acid transporter human serum albumin (HSA). The nitro group of NO₂-OA underwent electrochemical reduction at -0.75 V at pH 7.4 in an aqueous milieu. Based on observations of the R-NO₂ reduction process, the stability and reactivity of NO₂-OA was measured in comparison to oleic acid (OA) as the negative control. These electrochemically-based results were reinforced by computational quantum mechanical modeling. DFT calculations indicated that both the C9-NO₂ and C10-NO₂ positional isomers of NO₂

SUBMITTER: Zatloukalova M 

PROVIDER: S-EPMC6554544 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature