New UPLC-MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients.

ABSTRACT:

Aim

A rapid UPLC-MS/MS method for the determination of tamoxifen (TAM), N-desmethyltamoxifen, 4-hydroxytamoxifen and endoxifen in human plasma was validated, after a simple protein precipitation.

Materials and methods

The analysis was achieved on a C¹⁸ analytical column, using a gradient elution with a mobile phase of water and acetonitrile for 4.5 min.

Results

The validated method demonstrated good linearity between 1 and 500 ng/ml for TAM and N-desmethyltamoxifen; between 0.2 and 100 ng/ml for endoxifen and between 0.1 and 50 ng/ml for 4-hydroxytamoxifen. The method also provided satisfactory results in terms of within day and between day imprecisions and accuracy, and also in terms of time stability and specificity.

Conclusion

The me

SUBMITTER: Bobin-Dubigeon C 

PROVIDER: S-EPMC6554689 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature