Advancements in mitigating interference in quantitative polymerase chain reaction (qPCR) for microbial water quality monitoring.

ABSTRACT: The United States Environmental Protection Agency's (EPA)¹ 2012 Recreational Water Quality Criteria included an Enterococcus spp. quantitative polymerase chain reaction (qPCR) method as a supplemental indicator-method. In 2012, performance of qPCR for beach monitoring remained limited, specifically with addressing interference. A systematic literature search of peer-reviewed publications was conducted to identify where Enterococcus spp. and E. coli qPCR methods have been applied in ambient waters. In the present study, we evaluated interference rates, contributing factors resulting in increased interference in these methods, and method improvements that reduced interference. Information on qPCR methods of interest and interference controls were reported in 16 papers for Enteroco

SUBMITTER: Nappier SP 

PROVIDER: S-EPMC6555561 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature