Importance of potassium ions for ribosome structure and function revealed by long-wavelength X-ray diffraction.

ABSTRACT: The ribosome, the largest RNA-containing macromolecular machinery in cells, requires metal ions not only to maintain its three-dimensional fold but also to perform protein synthesis. Despite the vast biochemical data regarding the importance of metal ions for efficient protein synthesis and the increasing number of ribosome structures solved by X-ray crystallography or cryo-electron microscopy, the assignment of metal ions within the ribosome remains elusive due to methodological limitations. Here we present extensive experimental data on the potassium composition and environment in two structures of functional ribosome complexes obtained by measurement of the potassium anomalous signal at the K-edge, derived from long-wavelength X-ray diffraction data. We elucidate the role of potassium i

SUBMITTER: Rozov A 

PROVIDER: S-EPMC6555806 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature