APC/C^CDH1 synchronizes ribose-5-phosphate levels and DNA synthesis to cell cycle progression.

ABSTRACT: Accumulation of nucleotide building blocks prior to and during S phase facilitates DNA duplication. Herein, we find that the anaphase-promoting complex/cyclosome (APC/C) synchronizes ribose-5-phosphate levels and DNA synthesis during the cell cycle. In late G₁ and S phases, transketolase-like 1 (TKTL1) is overexpressed and forms stable TKTL1-transketolase heterodimers that accumulate ribose-5-phosphate. This accumulation occurs by asymmetric production of ribose-5-phosphate from the non-oxidative pentose phosphate pathway and prevention of ribose-5-phosphate removal by depleting transketolase homodimers. In the G₂ and M phases after DNA synthesis, expression of the APC/C adaptor CDH1 allows APC/C^CDH1 to degrade D-box-containing TKTL1, abrogating ribose-5-ph

SUBMITTER: Li Y 

PROVIDER: S-EPMC6555833 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature