Amlodipine induces vasodilation via Akt2/Sp1-activated miR-21 in smooth muscle cells.

ABSTRACT:

Background and purpose

The calcium antagonist amlodipine exerts important cardioprotective effects by modulating smooth muscle and endothelial functions. However, the mechanisms underlying these effects are incompletely understood.

Experimental approach

Western blotting was used to compare the expression of key genes involved in vascular smooth muscle cell (VSMC) phenotype conversion. Recombinant adeno-associated virus system was used to regulate miRNA expression in rats via tail vein. Bioinformatics was used to predict the transcriptional regulation of miR-21 upstream followed by biochemical validation using quantitative real-time polymerase chain reaction, ChIP-qPCR and EMSA assays.

Key results

Only the calcium antagonist amlodipine, and no other type of anti-hypert

SUBMITTER: Fang Q 

PROVIDER: S-EPMC6555864 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature