A mobile restriction modification system consisting of methylases on the IncA/C plasmid.

ABSTRACT:

Background

IncA/C plasmids play important roles in the development and dissemination of multidrug resistance in bacteria. These plasmids carry three methylase genes, two of which show cytosine specificity. The effects of such a plasmid on the host methylome were observed by single-molecule, real-time (SMRT) and bisulfite sequencing in this work.

Results

The results showed that the numbers of methylation sites on the host chromosomes were changed, as were the sequences recognized by MTase. The host chromosomes were completely remodified by the plasmid with a methylation pattern different from that of the host itself. When the three dcm genes were deleted, the transferability of the plasmid into other Vibrio cholerae and Escherichia coli strains was lost.

SUBMITTER: Wang R 

PROVIDER: S-EPMC6555945 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature