Codon usage optimization in pluripotent embryonic stem cells.

ABSTRACT:

Background

The uneven use of synonymous codons in the transcriptome regulates the efficiency and fidelity of protein translation rates. Yet, the importance of this codon bias in regulating cell state-specific expression programmes is currently debated. Here, we ask whether different codon usage controls gene expression programmes in self-renewing and differentiating embryonic stem cells.

Results

Using ribosome and transcriptome profiling, we identify distinct codon signatures during human embryonic stem cell differentiation. We find that cell state-specific codon bias is determined by the guanine-cytosine (GC) content of differentially expressed genes. By measuring the codon frequencies at the ribosome active sites interacting with transfer RNAs (tRNA), we further discover t

SUBMITTER: Bornelov S 

PROVIDER: S-EPMC6555954 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature