Genome-wide profiling of the alternative splicing provides insights into development in Plutella xylostella.

ABSTRACT:

Background

The diamondback moth (DBM), Plutella xylostella (L.), is a major pest of cruciferous crops worldwide. While the species has become a model for genomics, post-transcriptional mechanisms associated with development and sex determination have not been comprehensively studied and the lack of complete structure of mRNA transcripts limits further research.

Results

Here, we combined the methods of single-molecule long-read sequencing technology (IsoSeq) and RNA-seq to re-annotate the published DBM genome and present the genome-wide identification of alternative splicing (AS) associated with development and sex determination of DBM. In total, we identified ~ 13,900 genes (~ 77%) annotated in the DBM genome (version-2), resulting in the correction of 1586 wrongly annotated

SUBMITTER: Zhao Q 

PROVIDER: S-EPMC6556048 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature