Detection of unamplified target genes via CRISPR-Cas9 immobilized on a graphene field-effect transistor.

ABSTRACT: Most methods for the detection of nucleic acids require many reagents and expensive and bulky instrumentation. Here, we report the development and testing of a graphene-based field-effect transistor that uses clustered regularly interspaced short palindromic repeats (CRISPR) technology to enable the digital detection of a target sequence within intact genomic material. Termed CRISPR-Chip, the biosensor uses the gene-targeting capacity of catalytically deactivated CRISPR-associated protein 9 (Cas9) complexed with a specific single-guide RNA and immobilized on the transistor to yield a label-free nucleic-acid-testing device whose output signal can be measured with a simple handheld reader. We used CRISPR-Chip to analyse DNA samples collected from HEK293T cell lines expressing blue fluorescen

SUBMITTER: Hajian R 

PROVIDER: S-EPMC6556128 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature