Effect of Ocular Hypertension on D-β-Aspartic Acid-Containing Proteins in the Retinas of Rats.

ABSTRACT:

Purpose

To investigate the effect of ocular hypertension-induced isomerization of aspartic acid in retinal proteins.

Methods

Adult Wistar rats with ocular hypertension were used as an experimental model. D-β-aspartic acid-containing proteins were isolated by SDS-PAGE and western blot with an anti-D-β-aspartic acid antibody and identified by liquid chromatography-mass spectrometry analysis. The concentration of ATP was measured by ELISA.

Results

D-β-aspartic acid was expressed in a protein band at around 44.5 kDa at much higher quantities in the retinas of rats with ocular hypertension than in those of normotensive rats. The 44.5 kDa protein band was mainly composed of α-enolase, S-arrestin, and ATP synthase subunits α and β, i

SUBMITTER: Kanamoto T 

PROVIDER: S-EPMC6556240 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature