Sialic acid-binding immunoglobulin-like lectin (Siglec) 8 in patients with eosinophilic disorders: Receptor expression and targeting using chimeric antibodies.

ABSTRACT:

Background

Sialic acid-binding immunoglobulin-like lectin (Siglec) 8 is selectively expressed on eosinophils, mast cells, and basophils and, when engaged on eosinophils, can cause cell death.

Objective

We sought to characterize surface and soluble Siglec-8 (sSiglec-8) levels in normal donors (NDs) and eosinophilic donors (EOs) and assess the efficacy of anti-Siglec-8 antibodies in inducing eosinophil cell death in vitro.

Methods

Eosinophil expression of Siglec-8 was assessed by using flow cytometry and quantitative PCR. Serum sSiglec-8 levels were measured by means of ELISA. Induction of eosinophil death by IgG₄ (chimeric 2E2 IgG₄) and afucosylated IgG₁ (chimeric 2E2 IgG₁ [c2E2 IgG₁]) anti-Siglec-8 antibodies was

SUBMITTER: Legrand F 

PROVIDER: S-EPMC6556424 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature