REST-DRD2 mechanism impacts glioblastoma stem cell-mediated tumorigenesis.

ABSTRACT:

Background

Glioblastoma (GBM) is a lethal, heterogeneous human brain tumor, with regulatory mechanisms that have yet to be fully characterized. Previous studies have indicated that the transcriptional repressor REST (repressor element-1 silencing transcription factor) regulates the oncogenic potential of GBM stem cells (GSCs) based on level of expression. However, how REST performs its regulatory role is not well understood.

Methods

We examined 2 independent high REST (HR) GSC lines using genome-wide assays, biochemical validations, gene knockdown analysis, and mouse tumor models. We analyzed in-house patient tumors and patient data present in The Cancer Genome Atlas (TCGA).

Results

Genome-wide transcriptome and DNA-binding analyses suggested the dopamine receptor D2

SUBMITTER: Marisetty AL 

PROVIDER: S-EPMC6556851 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature