Development and validation of a high performance liquid chromatography method to determine nevirapine in plasma in a resource-limited setting.

ABSTRACT:

Background

There are several instances where nevirapine pharmacokinetic monitoring may be useful, such as in special populations or pharmacokinetic drug interaction studies that require the ascertainment of nevirapine pharmacokinetics in the sub-Saharan region.

Objectives

The main aim of this study was to produce a validated, sustainable and relevant nevirapine assay method that meets bio-analytical regulatory requirements.

Methods

The developed method utilised a Waters 2795 Alliance high performance liquid chromatography system with a 2996 photo diode array detector, an Atlantis dC18 5 micron, 3.9 mm × 150 mm analytical column and a gradient flow rate of 1 mL/min. Ultraviolet detection data were collected from 210 nm to 400 nm, extracted at 260 nm, and processed for

SUBMITTER: Makita-Chingombe F 

PROVIDER: S-EPMC6556864 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature