Small RNA Sequencing across Diverse Biofluids Identifies Optimal Methods for exRNA Isolation.

ABSTRACT: Poor reproducibility within and across studies arising from lack of knowledge regarding the performance of extracellular RNA (exRNA) isolation methods has hindered progress in the exRNA field. A systematic comparison of 10 exRNA isolation methods across 5 biofluids revealed marked differences in the complexity and reproducibility of the resulting small RNA-seq profiles. The relative efficiency with which each method accessed different exRNA carrier subclasses was determined by estimating the proportions of extracellular vesicle (EV)-, ribonucleoprotein (RNP)-, and high-density lipoprotein (HDL)-specific miRNA signatures in each profile. An interactive web-based application (miRDaR) was developed to help investigators select the optimal exRNA isolation method for their studies. miRDar provi

SUBMITTER: Srinivasan S 

PROVIDER: S-EPMC6557167 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature