Synthesis of 5'-Thio-3'-O-ribonucleoside Phosphoramidites.

ABSTRACT: The chemical synthesis of phosphoramidite derivatives of all four 5'-deoxy-5'-thioribonucleosides is described. These phosphoramidites contained trityl (A, G, C, and U), dimethoxytrityl (A and G), or tert-butyldisulfanyl (G) as the 5'-S-protecting group. The application of several of these phosphoramidites for solid-phase synthesis of oligoribonucleotides containing a 2'-O-photocaged 5'-S-phosphorothiolate linkage or 5'-thiol-labeled RNAs is also further investigated.

SUBMITTER: Li NS 

PROVIDER: S-EPMC6557410 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature