Characterization of 5-methylcytosine and 5-hydroxymethylcytosine in human placenta cell types across gestation.

ABSTRACT: The placenta is an important organ in pregnancy, however, very little is understood about placental development at a molecular level. This includes the role of epigenetic mechanisms and how they change throughout gestation. DNA methylation studies in this organ are complicated by the different cell types that make up the placenta, each with their own unique transcriptome and epigenome. Placental dysfunction is often associated with pregnancy complications such as preeclampsia (PE). Aberrant DNA methylation in the placenta has been identified in pregnancy complications. We used immunohistochemistry (IHC) and immunofluorescence (IF) to localize 5-methylcytosine (5-mC) and 5-hydroxymethylcytosine (5-hmC) in placenta tissue from first and second trimester as well as uncomplicated term and PE s

SUBMITTER: Wilson RL 

PROVIDER: S-EPMC6557593 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature