Differentiating between Inactive and Active States of Rhodopsin by Atomic Force Microscopy in Native Membranes.

ABSTRACT: Membrane proteins, including G protein-coupled receptors (GPCRs), present a challenge in studying their structural properties under physiological conditions. Moreover, to better understand the activity of proteins requires examination of single molecule behaviors rather than ensemble averaged behaviors. Force-distance curve-based AFM (FD-AFM) was utilized to directly probe and localize the conformational states of a GPCR within the membrane at nanoscale resolution based on the mechanical properties of the receptor. FD-AFM was applied to rhodopsin, the light receptor and a prototypical GPCR, embedded in native rod outer segment disc membranes from photoreceptor cells of the retina in mice. Both FD-AFM and computational studies on coarse-grained models of rhodopsin revealed that the active s

SUBMITTER: Senapati S 

PROVIDER: S-EPMC6557672 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature