MRI-guided robotic arm drives optogenetic fMRI with concurrent Ca²⁺ recording.

ABSTRACT: Optical fiber-mediated optogenetic activation and neuronal Ca²⁺ recording in combination with fMRI provide a multi-modal fMRI platform. Here, we developed an MRI-guided robotic arm (MgRA) as a flexible positioning system with high precision to real-time assist optical fiber brain intervention for multi-modal animal fMRI. Besides the ex vivo precision evaluation, we present the highly reliable brain activity patterns in the projected basal forebrain regions upon MgRA-driven optogenetic stimulation in the lateral hypothalamus. Also, we show the step-wise optical fiber targeting thalamic nuclei and map the region-specific functional connectivity with whole-brain fMRI accompanied by simultaneous calcium recordings to specify its circuit-specificity. The MgRA also guides the real-tim

SUBMITTER: Chen Y 

PROVIDER: S-EPMC6557837 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature