PacBio amplicon sequencing for metabarcoding of mixed DNA samples from lichen herbarium specimens.

ABSTRACT: The detection and identification of species of fungi in the environment using molecular methods heavily depends on reliable reference sequence databases. However, these databases are largely incomplete in terms of taxon coverage, and a significant effort is required from herbaria and living fungal collections for the mass-barcoding of well-identified and well-curated fungal specimens or strains. Here, a PacBio amplicon sequencing approach is applied to recent lichen herbarium specimens for the sequencing of the fungal ITS barcode, allowing a higher throughput sample processing than Sanger sequencing, which often required the use of cloning. Out of 96 multiplexed samples, a full-length ITS sequence of the target lichenised fungal species was recovered for 85 specimens. In addition, sequence

SUBMITTER: Gueidan C 

PROVIDER: S-EPMC6557899 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature