Optical second harmonic generation microscopy: application to the sensitive detection of cell membrane damage.

ABSTRACT: Optical second harmonic generation (SHG) is a nonlinear optical process which is sensitive to the symmetry of media. SHG microscopy allows for selective probing of a non-centrosymmetric area of sample. This type of nonlinear optical microscope was first used to observe ferroelectric domains and has been applied to various specimens including the biological samples to date. Imaging of the endogenous SHG of biological tissue has been utilized for the selective observation of filament systems in tissues such as collagen, myosin, and microtubules, which exhibit a polar structure. The cellular membrane can be selectively observed by the SHG microscope through membrane staining with amphiphilic polar dye molecules. It has been reported that, by imaging exogenous SHG of the membrane, sensitive de

SUBMITTER: Kato N 

PROVIDER: S-EPMC6557941 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature