Co-culture of Methanobrevibacter smithii with enterobacteria during urinary infection.

ABSTRACT:

Background

Urinary tract infections are known to be caused by bacteria, but the potential implications of archaea have never been studied in this context.

Methods

In two different university hospital centres we used specific laboratory methods for the detection and culture of archaeal methanogens in 383 urine specimens prospectively collected for diagnosing urinary tract infection (UTI).

Findings

Methanobrevibacter smithii was detected by quantitative PCR and sequencing in 34 (9%) of the specimens collected from 34 patients. Escherichia coli, Klebsiella pneumoniae, Enterobacter sp., Enterococcus faecium and mixed cultures were detected along with M. smithii in eighteen, six, three, one and six urine samples, respectively. Interestingly, using our specific culture meth

SUBMITTER: Grine G 

PROVIDER: S-EPMC6558020 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature