Ultrasensitive detection of cancer biomarkers by nickel-based isolation of polydisperse extracellular vesicles from blood.

ABSTRACT:

Background

Extracellular vesicles (EVs) are secreted membranous particles intensively studied for their potential cargo of diagnostic markers. Efficient and cost-effective isolation methods need to be established for the reproducible and high-throughput study of EVs in the clinical practice.

Methods

We designed the nickel-based isolation (NBI) to rapidly isolate EVs and combined it with newly-designed amplified luminescent proximity homogeneous assay or digital PCR to detect biomarkers of clinical utility.

Findings

From plasma of 46 healthy donors, we systematically recovered small EV (~250 nm of mean diameter; ~3 × 10¹⁰/ml) and large EV (~560 nm of mean diameter; ~5 × 10⁸/ml) lineages ranging from 50 to 700 nm, which displayed hematopoietic/endo

SUBMITTER: Notarangelo M 

PROVIDER: S-EPMC6558028 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature