Effects of Host, Sample, and in vitro Culture on Genomic Diversity of Pathogenic Mycobacteria.

ABSTRACT: Mycobacterium tuberculosis (M. tb), an obligate human pathogen and the etiological agent of tuberculosis (TB), remains a major threat to global public health. Comparative genomics has been invaluable for monitoring the emergence and spread of TB and for gaining insight into adaptation of M. tb. Most genomic studies of M. tb are based on single bacterial isolates that have been cultured for several weeks in vitro. However, in its natural human host, M. tb comprises complex, in some cases massive bacterial populations that diversify over the course of infection and cannot be wholly represented by a single genome. Recently, enrichment via hybridization capture has been used as a rapid diagnostic tool for TB, circumventing culturing protocols and enabl

SUBMITTER: Shockey AC 

PROVIDER: S-EPMC6558051 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature