Syncytin 1, CD9, and CD47 regulating cell fusion to form PGCCs associated with cAMP/PKA and JNK signaling pathway.

ABSTRACT:

Background

We have previously reported the formation of polyploid giant cancer cells (PGCCs) through endoreduplication or cell fusion after cobalt chloride (CoCl₂ ) induction. Cell fusion plays an important role in development and disease. However, the underlying molecular mechanism concerning cell fusion in PGCCs formation and clinicopathological significances remains unclear.

Methods

We treat HCT116 and LoVo cell with CoCl₂ and observed the cell fusion via fluorescent markers of different colors. Western blot and immunocytochemical staining were used to compare the expression and subcellular location of the fusion-related proteins syncytin 1, CD9, and CD47 along with PKA RIα, JNK1, and c-Jun between PGCCs and control cells from the HCT116 and LoVo cel

SUBMITTER: Fei F 

PROVIDER: S-EPMC6558479 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature