Characterization of internalin genes in Listeria monocytogenes from food and humans, and their association with the invasion of Caco-2 cells.

ABSTRACT:

Background

Internalins are surface proteins that are utilized by Listeria monocytogenes to facilitate its invasion into human intestinal epithelial cells. The expression of a full-length InlA is one of essential virulence factors for L. monocytogenes to cross the intestinal barrier in order to invade epithelial cells.

Results

In this study, the gene sequences of inlA in 120 L. monocytogenes isolates from food (n = 107) and humans (n = 13) were analyzed. Premature stop codon (PMSC) mutations in inlA were identified in 51 isolates (50 from food and 1 from human). Six mutation types of PMSCs were identified. Among the 51 isolates with PMSCs in inlA, there were 44 serogroup 1/2c, 3c isolates from food, of which seven belonged to serogroups 1/2a

SUBMITTER: Su X 

PROVIDER: S-EPMC6558679 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature