Agrobacterium-mediated vacuum infiltration and floral dip transformation of rapid-cycling Brassica rapa.

ABSTRACT:

Background

Rapid-cycling Brassica rapa (RCBr), also known as Wisconsin Fast Plants, are small robust plants with a short lifecycle that are widely used in biology teaching. RCBr have been used for decades but there are no published reports of RCBr genetic transformation. Agrobacterium-mediated vacuum infiltration has been used to transform pakchoi (Brassica rapa ssp. chinensis) and may be suitable for RCBr transformation. The floral dip transformation method, an improved version of vacuum infiltration, could make the procedure easier.

Results

Based on previous findings from Arabidopsis and pakchoi, plants of three different ages were inoculated with Agrobacterium. Kanamycin selection was suboptimal with RCBr; a GFP screen was used to identify candidate transformants. RCBr fl

SUBMITTER: Hu D 

PROVIDER: S-EPMC6558690 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature