Piezo1 induced apoptosis of type II pneumocytes during ARDS.

ABSTRACT:

Objective

The mechanisms of lung injury in acute respiratory distress syndrome (ARDS) are not well understood.Piezo1 was recently identified as a mechanotransduction protein. The present study found the expression of Piezo1 in type II pneumocytes and investigated its role in mediating ARDS-related lung injury.

Methods

Sprague-Dawley rats were used to establish an ARDS model, the expression of Piezo1,lung injuries, apoptosis as well as calcium influx were assessed.

Results

Piezo1 was expressed in type II pneumocytes as shown by immunofluorescence staining and expression was increased in the ARDS model. Knockdown of Piezo1 reduced apoptosis which was related to the elevation of Bcl-2.Calcium influx played a vital role in Piezo1-induced apoptosis.

Conclusion

Piezo

SUBMITTER: Liang GP 

PROVIDER: S-EPMC6558715 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature