Single-photon emission computed tomography/computed tomography imaging of RAGE in smoking-induced lung injury.

ABSTRACT:

Background

Expression of the Receptor for Advanced Glycation Endproducts (RAGE) initiates pro-inflammatory pathways resulting in lung destruction. We hypothesized that RAGE directed imaging demonstrates increased lung uptake in smoke-exposure.

Methods

After exposure to room air or to cigarette smoke for 4-weeks or 16-weeks, rabbits were injected with ^99mTc-anti-RAGE F(ab')₂ and underwent Single-Photon Emission Computed Tomography/Computed Tomography (SPECT/CT) imaging. Lung radiotracer uptake was calculated as percent injected dose (%ID). Lungs were dissected for gamma well counting and histological analysis.

Results

^99mTc-anti-RAGE F(ab')₂ SPECT/CT imaging demonstrated increased lung expression of RAGE with smoke exposure c

SUBMITTER: Goldklang MP 

PROVIDER: S-EPMC6558785 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature