Silencing of ANKRD12 circRNA induces molecular and functional changes associated with invasive phenotypes.

ABSTRACT:

Background

Circular RNAs (circRNAs) that form through non-canonical backsplicing events of pre-mRNA transcripts are evolutionarily conserved and abundantly expressed across species. However, the functional relevance of circRNAs remains a topic of debate.

Methods

We identified one of the highly expressed circRNA (circANKRD12) in cancer cell lines and characterized it validated it by Sanger sequencing, Real-Time PCR. siRNA mediated silencing of the circular junction of circANKRD12 was followed by RNA Seq analysis of circANKRD12 silenced cells and control cells to identify the differentially regulated genes. A series of cell biology and molecular biology techniques (MTS assay, Migration analysis, 3D organotypic models, Real-Time PCR, Cell cycle analysis, Western blot analysis,

SUBMITTER: Karedath T 

PROVIDER: S-EPMC6558796 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature