The activin-follistatin anti-inflammatory cycle is deregulated in synovial fibroblasts.

ABSTRACT:

Background

Activin A and follistatin exhibit immunomodulatory functions, thus affecting autoinflammatory processes as found in rheumatoid arthritis (RA). The impact of both proteins on the behavior of synovial fibroblasts (SF) in RA as well as in osteoarthritis (OA) is unknown.

Methods

Immunohistochemical analyses of synovial tissue for expression of activin A and follistatin were performed. The influence of RASF overexpressing activin A on cartilage invasion in a SCID mouse model was examined. RASF and OASF were stimulated with either IL-1β or TNFα in combination with or solely with activin A, activin AB, or follistatin. Protein secretion was measured by ELISA and mRNA expression by RT-PCR. Smad signaling was confirmed by western blot.

Results

In human RA synovial ti

SUBMITTER: Diller M 

PROVIDER: S-EPMC6558802 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature