WellInverter: a web application for the analysis of fluorescent reporter gene data.

ABSTRACT:

Background

Fluorescent reporter genes have become widely used for monitoring gene expression in living cells. When a microbial strain carrying a reporter gene is grown in a microplate reader, the fluorescence and the absorbance (optical density) of the culture can be automatically measured every few minutes in a highly parallelized way. The extraction of useful information from the resulting large amounts of data is not easy to achieve, because the fluorescence and absorbance measurements are only indirectly related to promoter activities and protein concentrations, requiring mathematical models of the expression of reporter genes for their interpretation. Although the principles of the analysis of reporter gene data are well-established today, there is a lack of general-purpose bi

SUBMITTER: Martin Y 

PROVIDER: S-EPMC6558888 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature