Non-invasive, ratiometric determination of intracellular pH in Pseudomonas species using a novel genetically encoded indicator.

ABSTRACT: The ability of Pseudomonas species to thrive in all major natural environments (i.e. terrestrial, freshwater and marine) is based on its exceptional capability to adapt to physicochemical changes. Thus, environmental bacteria have to tightly control the maintenance of numerous physiological traits across different conditions. The intracellular pH (pH_i ) homoeostasis is a particularly important feature, since the pH_i influences a large portion of the biochemical processes in the cell. Despite its importance, relatively few reliable, easy-to-implement tools have been designed for quantifying in vivo pH_i changes in Gram-negative bacteria with minimal manipulations. Here we describe a convenient, non-invasive protocol for the quantification of the pH_i

SUBMITTER: Arce-Rodriguez A 

PROVIDER: S-EPMC6559197 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature