Pretreatment of Miscanthus with biomass-degrading bacteria for increasing delignification and enzymatic hydrolysability.

ABSTRACT: Biomass recalcitrance is still a main challenge for the production of biofuels and high-value products. Here, an alternative Miscanthus pretreatment method by using lignin-degrading bacteria was developed. Six efficient Miscanthus-degrading bacteria were first cultured to produce laccase by using 0.5% Miscanthus biomass as carbon source. After 1-5 days of incubation, the maximum laccase activities induced by Miscanthus in the six strains were ranged from 103 to 8091 U l^-1 . Then, the crude enzymes were directly diluted by equal volumes of citrate buffer and added Miscanthus biomass to a solid concentration at 4% (w/v). The results showed that all bacterial pretreatments significantly decreased the lignin content, especially in the presence of two laccase mediators (ABTS and HBT)

SUBMITTER: Guo H 

PROVIDER: S-EPMC6559207 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature