Microglial proliferation and monocyte infiltration contribute to microgliosis following status epilepticus.

ABSTRACT: Microglial activation has been recognized as a major contributor to inflammation of the epileptic brain. Seizures are commonly accompanied by remarkable microgliosis and loss of neurons. In this study, we utilize the CX3CR1^GFP/+ CCR2^RFP/+ genetic mouse model, in which CX3CR1⁺ resident microglia and CCR2⁺ monocytes are labeled with GFP and RFP, respectively. Using a combination of time-lapse two-photon imaging and whole-cell patch clamp recording, we determined the distinct morphological, dynamic, and electrophysiological characteristics of infiltrated monocytes and resident microglia, and the evolution of their behavior at different time points following kainic acid-induced seizures. Seizure activated microglia presented enlarged somas with less

SUBMITTER: Feng L 

PROVIDER: S-EPMC6559368 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature