The Relationship Between rs3212986C>A Polymorphism and Tumor Stage in Lung Cancer Patients.

ABSTRACT: Background The nucleotide excision repair (NER) system is one of the most important deoxyribonucleic acid (DNA) repair mechanisms and is critical for chemotherapy resistance. We conducted the present study to investigate the association between two polymorphisms of excision of repair cross-complementing group 1 (ERCC1), the key component of the NER pathway, and the clinicopathological features of patients with non-small cell lung cancer (NSCLC). Methods A total of 38 patients with confirmed NSCLC were included in our study. DNA was extracted from peripheral blood. ERCC1 rs3212986 (8092) and rs11615 (118) were genotyped using molecular assays including polymerase chain reaction (PCR) with restriction fragment length polymorphism (by MboII and HpyCH4 enzymes) and sequencing. Results The PCR

SUBMITTER: Anoushirvani AA 

PROVIDER: S-EPMC6559387 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature