A new cis-acting regulatory element driving gene expression in the zebrafish pineal gland.

ABSTRACT:

Motivation

The identification of functional cis-acting DNA regulatory elements is a crucial step towards understanding gene regulation. Ab initio motif detection algorithms have been extensively used in search of regulatory elements. Yet, their success in providing experimentally validated regulatory elements in vertebrates has been limited.

Results

Here we report in silico identification and in vivo validation of regulatory elements that determine enhanced gene expression in the pineal gland of zebrafish. Microarray data enabled detection of genes that exhibit high expression in the pineal gland. The promoter regions of these genes were computationally analyzed in order to identify overrepresented motifs. The highest ranking motif identified is a CRX/OTX binding site, known

SUBMITTER: Alon S 

PROVIDER: S-EPMC6559713 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature