Glucose controls glucagon secretion by directly modulating cAMP in alpha cells.

ABSTRACT:

Aims/hypothesis

Glucagon is critical for normal glucose homeostasis and aberrant secretion of the hormone aggravates dysregulated glucose control in diabetes. However, the mechanisms by which glucose controls glucagon secretion from pancreatic alpha cells remain elusive. The aim of this study was to investigate the role of the intracellular messenger cAMP in alpha-cell-intrinsic glucose regulation of glucagon release.

Methods

Subplasmalemmal cAMP and Ca²⁺ concentrations were recorded in isolated and islet-located alpha cells using fluorescent reporters and total internal reflection microscopy. Glucagon secretion from mouse islets was measured using ELISA.

Results

Glucose induced Ca²⁺-independent alterations of the subplasmalemmal cAMP concentrati

SUBMITTER: Yu Q 

PROVIDER: S-EPMC6560012 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature