Nerve growth factor gene therapy improves bone marrow sensory innervation and nociceptor-mediated stem cell release in a mouse model of type 1 diabetes with limb ischaemia.

ABSTRACT:

Aims/hypothesis

Sensory neuropathy is common in people with diabetes; neuropathy can also affect the bone marrow of individuals with type 2 diabetes. However, no information exists on the state of bone marrow sensory innervation in type 1 diabetes. Sensory neurons are trophically dependent on nerve growth factor (NGF) for their survival. The aim of this investigation was twofold: (1) to determine if sensory neuropathy affects the bone marrow in a mouse model of type 1 diabetes, with consequences for stem cell liberation after tissue injury; and (2) to verify if a single systemic injection of the NGF gene exerts long-term beneficial effects on these phenomena.

Methods

A mouse model of type 1 diabetes was generated in CD1 mice by administration of streptozotocin; vehicle was a

SUBMITTER: Dang Z 

PROVIDER: S-EPMC6560027 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature