Effects of Different Diets on Microbiota in The Small Intestine Mucus and Weight Regulation in Rats.

ABSTRACT: While the microbial community of the small intestine mucus (SIM) may also play a role in human health maintenance and disease genesis, it has not been extensively profiled and whether it changes with diet is still unclear. To investigate the flora composition of SIM and the effects of diet on it, we fed SD rats for 12 weeks with standard diet (STD), high-fat diet (HFD), high-sugar diet (HSD) and high-protein diet (HPD), respectively. After 12 weeks, the rats were sacrificed, SIM and stool samples were collected, and high-throughput 16S rRNA gene sequencing was used to analyze the microbiota. We found that fecal microbiota (FM) was dominated by Firmicutes and Bacteroidetes, while in SIM, Firmicutes and Proteobacteria were the two most abundant phyla and the level of Bacteroidetes dramatical

SUBMITTER: Meng Y 

PROVIDER: S-EPMC6560036 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature