Benefits of using genomic insulators flanking transgenes to increase expression and avoid positional effects.

ABSTRACT: For more than 20 years, plant biologists have tried to achieve complete control of transgene expression. Until the techniques to target transgenes to safe harbor sites in the genome become routine, flanking transgenes with genetic insulators, DNA sequences that create independent domains of gene expression, can help avoid positional effects and stabilize their expression. We have, for the first time, compared the effect of three insulator sequences previously described in the literature and one never tested before. Our results indicate that their use increases transgene expression, but only the last one reduces variability between lines and between individuals. We have analyzed the integration of insulator-flanked T-DNAs using whole genome re-sequencing (to our knowledge, also for the firs

SUBMITTER: Perez-Gonzalez A 

PROVIDER: S-EPMC6560062 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature