Acetate Metabolism and the Inhibition of Bacterial Growth by Acetate.

ABSTRACT: During aerobic growth on glucose, Escherichia coli excretes acetate, a mechanism called "overflow metabolism." At high concentrations, the secreted acetate inhibits growth. Several mechanisms have been proposed for explaining this phenomenon, but a thorough analysis is hampered by the diversity of experimental conditions and strains used in these studies. Here, we describe the construction of a set of isogenic strains that remove different parts of the metabolic network involved in acetate metabolism. Analysis of these strains reveals that (i) high concentrations of acetate in the medium inhibit growth without significantly perturbing central metabolism; (ii) growth inhibition persists even when acetate assimilation is completely blocked; and (iii) regulatory interactions mediated b

SUBMITTER: Pinhal S 

PROVIDER: S-EPMC6560135 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature