In Vitro Activation of Seed-Transmitted Cultivation-Recalcitrant Endophytic Bacteria in Tomato and Host⁻Endophyte Mutualism.

ABSTRACT: This study was aimed at exploring seed transmission of endophytic bacteria in tomato utilizing aseptic in vitro conditions. Cultivation-based studies were undertaken on two tomato cultivars "Arka Vikas" and "Arka Abha" employing surface sterilized seeds, aseptically germinated seeds and in vitro grown seedlings at different stages. Bacillus sp. appeared primarily as seed externally-associated bacteria. Tissue homogenate from extensively surface-sterilized seeds, day-3 germinating seeds, or 10-day in vitro seedlings did not show any cultivable bacteria on two bacteriological media. Indexing of 4-week old healthy seedlings with seed-coat removal following seed germination showed bacterial association in 50-75% seedlings yielding 10⁶-10⁷ cfu g^-1 tissues

SUBMITTER: Shaik SP 

PROVIDER: S-EPMC6560416 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature