DNA methylation profiling allows for characterization of atrial and ventricular cardiac tissues and hiPSC-CMs.

ABSTRACT:

Background

Cardiac disease modelling using human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) requires thorough insight into cardiac cell type differentiation processes. However, current methods to discriminate different cardiac cell types are mostly time-consuming, are costly and often provide imprecise phenotypic evaluation. DNA methylation plays a critical role during early heart development and cardiac cellular specification. We therefore investigated the DNA methylation pattern in different cardiac tissues to identify CpG loci for further cardiac cell type characterization.

Results

An array-based genome-wide DNA methylation analysis using Illumina Infinium HumanMethylation450 BeadChips led to the identification of 168 differentially methylated CpG loc

SUBMITTER: Hoff K 

PROVIDER: S-EPMC6560887 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature