Cardiac myosin activation with 2-deoxy-ATP via increased electrostatic interactions with actin.

ABSTRACT: The naturally occurring nucleotide 2-deoxy-adenosine 5'-triphosphate (dATP) can be used by cardiac muscle as an alternative energy substrate for myosin chemomechanical activity. We and others have previously shown that dATP increases contractile force in normal hearts and models of depressed systolic function, but the structural basis of these effects has remained unresolved. In this work, we combine multiple techniques to provide structural and functional information at the angstrom-nanometer and millisecond time scales, demonstrating the ability to make both structural measurements and quantitative kinetic estimates of weak actin-myosin interactions that underpin sarcomere dynamics. Exploiting dATP as a molecular probe, we assess how small changes in myosin structure translate to electro

SUBMITTER: Powers JD 

PROVIDER: S-EPMC6561254 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature